Mangkornkanok-Mark M, Eisenstein R, Bahu R M
J Histochem Cytochem. 1981 Apr;29(4):547-52. doi: 10.1177/29.4.7019307.
Rabbit antisera prepared against bovine cartilage and aortic proteoglycan monomer (PGM) were employed to identify and localize the distribution and cross-species reactivities of these PGMs. A specific reaction to both cartilage and aortic PGM was obtained as revealed by rocket immunoelectrophoresis of the immunogens against the respective antisera, with no reaction against bovine serum albumin or human fibronectin. These antisera appear to demonstrate tissue specificity. Indirect immunofluorescence studies with antiserum to aortic PGM on bovine aorta tissue revealed intense localization of fluorescence in the intima and superficial media with staining alone collagen fibers, around smooth muscle cells, and on the surface of elastin. When antiserum to cartilage PGM was applied to bovine aorta, staining was much weaker and localized more in the interfibrillar matrix. On the other hand, when antiserum to aortic PGM was used to stain bovine nasal septum cartilage, fluorescent staining was restricted to the pericellular matrix, in contrast to the diffuse, intense staining of both pericellular and extraterritorial staining by antiserum to cartilage PGM. Absorption with eigher bovine aorta or cartilage PGM abolished antibody activity against the absorbing molecules only. The immunologic distinction between the pericellular and extraterritorial cartilage matrix was demonstrated further when both antisera were studied with guanidine chloride (GuHCl)-extracted bovine nasal septum cartilage, when they both demonstrated the same pericellular matrix staining. Despite the tissue specificity, these antisera are not completely species specific, since staining in a similar pattern was observed in both human and rat tissues.
制备了针对牛软骨和主动脉蛋白聚糖单体（PGM）的兔抗血清，用于鉴定和定位这些PGM的分布及种间反应性。通过将免疫原与相应抗血清进行火箭免疫电泳显示，对软骨和主动脉PGM均获得了特异性反应，而对牛血清白蛋白或人纤连蛋白无反应。这些抗血清似乎具有组织特异性。用主动脉PGM抗血清对牛主动脉组织进行间接免疫荧光研究显示，荧光强烈定位于内膜和浅表中膜，仅在胶原纤维周围、平滑肌细胞周围以及弹性蛋白表面有染色。当将软骨PGM抗血清应用于牛主动脉时，染色要弱得多，且更多地定位于纤维间基质。另一方面，当用主动脉PGM抗血清对牛鼻中隔软骨进行染色时，荧光染色仅限于细胞周围基质，而软骨PGM抗血清对细胞周围和域外基质均有弥漫、强烈的染色。用牛主动脉或软骨PGM吸收仅消除了针对吸收分子的抗体活性。当用氯化胍（GuHCl）提取的牛鼻中隔软骨对两种抗血清进行研究时，进一步证明了细胞周围和域外软骨基质之间的免疫学差异，此时它们均显示相同的细胞周围基质染色。尽管具有组织特异性，但这些抗血清并非完全种属特异性，因为在人和大鼠组织中均观察到类似的染色模式。
