Mera S L, Lovett F, Davies J D
Histochem J. 1985 Feb;17(2):243-58. doi: 10.1007/BF01003223.
Antibodies to alpha-elastin peptides, amyloid P component, lysozyme and plasma protease inhibitors have been used in an immunoperoxidase method to stain elastic fibres in frozen sections of human breast tissues. A loss of immunoreactivity seen in formalin-fixed, paraffin-embedded sections was reversed by a preliminary proteolysis. Differences in the tinctorial dye and immunohistochemical staining patterns following proteolysis by a variety of enzymes suggests a selective unmasking or removal of elastic fibre components and thus the presence of separate binding sites for individual antibodies and tinctorial dyes. Antibody blocking experiments and double immunoenzymatic labelling support the existence of several different epitopes within elastic fibres.
针对α-弹性蛋白肽、淀粉样P成分、溶菌酶和血浆蛋白酶抑制剂的抗体已被用于免疫过氧化物酶法，以对人乳腺组织冰冻切片中的弹性纤维进行染色。在福尔马林固定、石蜡包埋切片中观察到的免疫反应性丧失可通过初步蛋白酶解得以恢复。多种酶进行蛋白酶解后，在染色染料和免疫组织化学染色模式上的差异表明弹性纤维成分存在选择性的暴露或去除，因此存在针对个别抗体和染色染料的不同结合位点。抗体阻断实验和双免疫酶标记支持弹性纤维内存在几种不同的表位。
