Yeh C J, Hsi B L, Faulk W P
J Immunol Methods. 1981;43(3):269-75. doi: 10.1016/0022-1759(81)90174-5.
The use of a fluorescence method employing propidium iodide to determine nuclear morphology of different cell types by epi-illumination in membrane immunofluorescence is described. Its usefulness is illustrated by differentiating nucleated and anuclear transferrin receptor-bearing cells as well as by simultaneously determining the viability of cell suspension without requiring a change from fluorescence microscopy, this causing no loss of visual accommodation.
本文描述了一种利用碘化丙啶的荧光方法，通过落射照明在膜免疫荧光中确定不同细胞类型的核形态。通过区分有核和无核的转铁蛋白受体细胞以及同时确定细胞悬液的活力，说明了该方法的实用性，且无需从荧光显微镜转换，不会导致视觉调节能力的丧失。
