Ockleford C D, Hsi B L, Wakely J, Badley R A, Whyte A, Faulk W P
J Immunol Methods. 1981;43(3):261-7. doi: 10.1016/0022-1759(81)90173-3.
Some examples are given of immunofluorescence with tissue sections and microtubular cytoskeletons of cultured cells where the fluorescent dye propidium iodide (PI) has been used as marker of nuclei. The emission wave length of IP is longer than that of fluorescein, making it possible to use several different and commonly available filter combinations. The use of nuclei as positional indicators is often a more suitable method than phase microscopy combined with immunofluorescence because of low background illumination against which morphology is viewed, circumventing the need for often expensive phase optics.
文中给出了一些利用组织切片和培养细胞微管细胞骨架进行免疫荧光检测的示例，其中使用荧光染料碘化丙啶（PI）作为细胞核的标记物。碘化丙啶的发射波长比荧光素长，这使得使用几种不同且常用的滤光片组合成为可能。将细胞核用作位置指示物通常是一种比相差显微镜结合免疫荧光更合适的方法，因为观察形态时背景照明较低，从而无需使用通常较为昂贵的相差光学元件。
