Krapoth Tim Christopher, Henle Gina Sophie, Avdyli Mihrije, Bektić Berina, Schwarzkopf Katharina Maria, Bešić Larisa, Zeuzem Stefan, Welsch Christoph, Kraus Nico, Ortiz Cristina
Goethe University, Frankfurt University Hospital, Medical Clinic 1, 60596 Frankfurt, Germany.
Department of Genetics and Bioengineering, International Burch University, Francuske Revolucije bb, 71210 Sarajevo, Bosnia and Herzegovina.
Int J Mol Sci. 2024 Dec 17;25(24):13521. doi: 10.3390/ijms252413521.
This study demonstrates the effectiveness of propidium iodide as a reliable marker for detecting dead or dying cells in frozen liver tissue sections. By comparing propidium iodide staining with the widely used Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, both methods showed consistent results in disease models such as alcohol-induced fibrosis and Western diet-induced fatty liver. Additionally, propidium iodide was successfully co-stained with other fluorescent markers, like phalloidin (for actin filaments) and antibodies targeting collagen, enabling detailed spatial analysis of dying cells within tissue. This multiplex approach allows for a deeper understanding of tissue organization and cell death localization, particularly in complex conditions like liver fibrosis. Moreover, our results suggest that propidium iodide staining can be applied beyond current models, offering a more accessible and cost-effective alternative to traditional methods, like TUNEL. Furthermore, its integration with other markers enables simultaneous analysis of immune responses and tissue damage, making it a powerful tool for future studies on liver disease and other inflammatory conditions. This technique has the potential to advance research into disease mechanisms and improve the evaluation of novel therapeutic strategies targeting tissue regeneration and inflammation control.
本研究证明了碘化丙啶作为检测冷冻肝组织切片中死亡或濒死细胞的可靠标志物的有效性。通过将碘化丙啶染色与广泛使用的末端脱氧核苷酸转移酶dUTP缺口末端标记法（TUNEL）进行比较，两种方法在酒精性纤维化和西式饮食诱导的脂肪肝等疾病模型中均显示出一致的结果。此外，碘化丙啶成功地与其他荧光标记物共同染色，如鬼笔环肽（用于肌动蛋白丝）和靶向胶原蛋白的抗体，从而能够对组织内的濒死细胞进行详细的空间分析。这种多重方法有助于更深入地了解组织结构和细胞死亡定位，特别是在肝纤维化等复杂情况下。此外，我们的结果表明，碘化丙啶染色可应用于当前模型之外，为TUNEL等传统方法提供了一种更易于使用且成本效益更高的替代方法。此外，它与其他标记物的整合能够同时分析免疫反应和组织损伤，使其成为未来肝病和其他炎症性疾病研究的有力工具。这项技术有可能推动对疾病机制的研究，并改善针对组织再生和炎症控制的新型治疗策略的评估。
