Jäger G, Dörmer P
J Immunol Methods. 1981;44(1):55-62. doi: 10.1016/0022-1759(81)90106-x.
The reaction product of the unlabeled antibody enzyme method was quantitated at the single cell level by absorption microphotometry. Attempts were made to demonstrate that the amount of dye deposited on individual cells can be taken as a measure of the number of antibody molecules bound. In an experiment with prefixed sheep erythrocytes loaded in excess with amboceptor a close relationship was observed between cell size and the amount of dye measured. Furthermore, the known differences in A1 antigen density on human A1 and A2 erythrocytes were satisfactorily reproduced with this quantitative approach. As regards T antigen density on human lymphocytes of different origin, the results presented tally with data in the literature. It is therefore concluded that the method is suitable for quantitating antibody amounts bound to individual cells.
采用吸收显微光度法在单细胞水平对未标记抗体酶法的反应产物进行定量。试图证明沉积在单个细胞上的染料量可作为结合抗体分子数量的一种度量。在一项实验中，对预先固定并过量加载调理素的绵羊红细胞进行观察，发现细胞大小与所测染料量之间存在密切关系。此外，用这种定量方法令人满意地再现了人类A1和A2红细胞上已知的A1抗原密度差异。关于不同来源人类淋巴细胞上的T抗原密度，所呈现的结果与文献中的数据相符。因此得出结论，该方法适用于定量与单个细胞结合的抗体量。
