Esber H J, DeCourcy S J, Bogden A E
J Immunopharmacol. 1981;3(1):79-92. doi: 10.3109/08923978109026420.
The immunopotentiating activity of staphage lysate (SPL) was evaluated in terms of its immune protection against lethal bacterial infection and its antitumor activity. Mice were pretreated weekly with 10(8) viable, Staphylococcus aureus, strain 18Z for 3 weeks (Induction), followed by intraperitoneal SPL injections (Elicitation) at various times in relation to infectious challenge or tumor implantation. Induction without elicitation, or elicitation alone failed to provide protection against Klebsiella pneumoniae infection and resulted in only 30-40% survival against homologous infection with pathogenic S. aureus type III, whereas combined induction and elicitation produced enhanced resistance induction and elicitation regimens resulted in 50% and 80-100% survival in mice inoculated with K. pneumoniae and S. aureus, respectively. SPL had no antitumor effect in mice implanted with median survival time resulting from induction and elicitation in animals implanted which Ehrlich's ascites. This enhancement of immune resistance may possibly be related to activation of thymus-modulated lymphocytes and macrophages by SPL.
通过葡萄球菌噬菌体裂解物（SPL）对致死性细菌感染的免疫保护作用及其抗肿瘤活性来评估其免疫增强活性。小鼠每周用10⁸个活的金黄色葡萄球菌18Z菌株预处理3周（诱导），随后在与感染攻击或肿瘤植入相关的不同时间进行腹腔注射SPL（激发）。仅诱导而无激发，或仅激发均不能提供针对肺炎克雷伯菌感染的保护，并且在抵抗III型致病性金黄色葡萄球菌的同源感染时存活率仅为30 - 40%，而诱导和激发相结合则产生增强的抵抗力，在接种肺炎克雷伯菌和金黄色葡萄球菌的小鼠中，诱导和激发方案分别导致50%和80 - 100%的存活率。在植入艾氏腹水瘤的动物中，由诱导和激发导致的中位生存时间来看，SPL对其没有抗肿瘤作用。这种免疫抵抗力的增强可能与SPL激活胸腺调节的淋巴细胞和巨噬细胞有关。
