Esber H J, Ganfield D, Rosenkrantz H
Immunopharmacology. 1985 Oct;10(2):77-82. doi: 10.1016/0162-3109(85)90032-3.
The immunological enhancing activity of staphage lysate on the primary immune response of mice as reflected by specific heteroantibody production to a single antigenic stimulus and on immunoglobulin synthesis was determined. Staphage lysate was administered at different periods in relation to time of sheep erythrocyte injection so that both the inductive and the productive phase of the immune response could be evaluated. Treatment with staphage lysate induced pronounced enhancement of the hemagglutinin response by day 14 regardless of the dose or the time of its administration. Significantly higher antibody levels in the test groups were observed when compared with the control animals, peak antibody titers being 21 days following antigenic challenge. Furthermore, staphage lysate administered without an accompanying antigen evoked a heightened serum immunoglobulin level in mice for a period of more than 14 days after injection. IgG1, IgG2a and IgG2b were significantly elevated as a direct result of this treatment. This increased output of immunoglobulin synthesis and heightened hemagglutinin titers demonstrate that staphage lysate is not only an immunomodulator of cell-mediated immunity, as previously reported, but also an effective immunoadjuvant of the humoral antibody response capability in the host.
测定了葡萄球菌噬菌体裂解物对小鼠初次免疫反应的免疫增强活性，该活性通过对单一抗原刺激产生的特异性异种抗体生成以及免疫球蛋白合成来反映。在与绵羊红细胞注射时间相关的不同时期给予葡萄球菌噬菌体裂解物，以便评估免疫反应的诱导期和产生期。无论剂量或给药时间如何，在第14天时，用葡萄球菌噬菌体裂解物处理均能显著增强血凝素反应。与对照动物相比，试验组中观察到抗体水平显著更高，抗原攻击后21天达到抗体滴度峰值。此外，在无伴随抗原的情况下给予葡萄球菌噬菌体裂解物，在注射后14天以上的时间内可使小鼠血清免疫球蛋白水平升高。作为该处理的直接结果，IgG1、IgG2a和IgG2b显著升高。免疫球蛋白合成的这种增加以及血凝素滴度的升高表明，葡萄球菌噬菌体裂解物不仅如先前报道的那样是细胞介导免疫的免疫调节剂，而且还是宿主体液抗体反应能力的有效免疫佐剂。
