小鼠巨噬细胞中吞噬体-溶酶体融合的调节
Modulation of phagosome-lysosome fusion in mouse macrophages.
作者信息
Kielian M C, Cohn Z A
出版信息
J Exp Med. 1981 Apr 1;153(4):1015-20. doi: 10.1084/jem.153.4.1015.
Abstract
A previously described fluorescence assay has been used to characterize factors that modulate phagosome-lysosome (P-L) fusion in mouse macrophages. Fusion was not affected by enzymatic modification or by concanavalin A cross-linking of the plasma membrane or by coating the phagocytic particle with concanavalin A or immune serum. Pretreatment of cells with 10-5-10-4 M colchicine, or treatment immediately after ingestion with 1-10 microgram/ml cytochalasin did not alter P-L fusion; implying that the cytoskeleton does not control fusion in a rate-limiting way. Fusion was strikingly elevated in 5-h cultures of activated macrophages from immune-boosted mice. A lower enhancement was seen in cells activated by proteose-peptone, a nonspecific inflammatory agent.
摘要
一种先前描述的荧光测定法已被用于表征调节小鼠巨噬细胞中吞噬体-溶酶体（P-L）融合的因子。融合不受酶修饰、质膜的伴刀豆球蛋白A交联或用伴刀豆球蛋白A或免疫血清包被吞噬颗粒的影响。用10^-5 - 10^-4 M秋水仙碱预处理细胞，或在摄取后立即用1 - 10微克/毫升细胞松弛素处理，均未改变P-L融合；这意味着细胞骨架并非以限速方式控制融合。在来自免疫增强小鼠的活化巨噬细胞的5小时培养物中，融合显著升高。在由蛋白胨（一种非特异性炎症剂）激活的细胞中观察到较低程度的增强。
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