Edwards J C, Sedgwick A D, Willoughby D A
J Pathol. 1981 Jun;134(2):147-56. doi: 10.1002/path.1711340205.
We have attempted to verify the suggestion that synovial membrane is the result of mechanical disruption of connective tissue, and may occur at any site. Mechanical disruption of the subcutaneous connective tissue was achieved in rats and mice by the repeated injection of air. The resulting cavities developed a lining structure with many of the features of synovial membrane as judged by electron microscopy, and light microscopy using haematoxylin and eosin and van Gieson stains, esterase activity and immunofluorescent staining for Ia antigen. A structure closely resembling synovium is formed as early as 6 days, providing a convenient method for studying large quantities of facsimile synovial tissue under a wide variety of easily administered stimuli.
我们试图验证滑膜是结缔组织机械性破坏的结果且可能发生在任何部位这一观点。通过在大鼠和小鼠体内反复注射空气实现皮下结缔组织的机械性破坏。通过电子显微镜、苏木精-伊红染色和范吉森染色的光学显微镜、酯酶活性以及Ia抗原免疫荧光染色判断，由此形成的腔隙产生了具有滑膜许多特征的衬里结构。早在6天时就形成了与滑膜极为相似的结构，这为在各种易于施加的刺激下研究大量类似滑膜组织提供了一种便利方法。
