Smith J S, Karow A M
Res Commun Chem Pathol Pharmacol. 1980 Dec;30(3):459-68.
Islets of Langerhans isolated by collagenase digestion were shown to elicit an immediate monophasic release by insulin when subjected to cryoprotective concentrations of dimethyl sulfoxide (Me2SO) in a perifusion system. This response only terminated after removal of the drug from the perifusate, and was attributed to membrane permeability changes as demonstrated by the immediate monophasic release of phosphate from perifused islets which also terminated only after removal of the drug.
通过胶原酶消化分离出的胰岛在灌注系统中受到冷冻保护浓度的二甲基亚砜（Me2SO）作用时，会引发胰岛素的即时单相释放。这种反应只有在从灌注液中去除药物后才会终止，并且被归因于膜通透性的变化，这一点通过灌注胰岛中磷酸盐的即时单相释放得到证明，该释放也只有在去除药物后才会终止。
