Williams M R, Bunch K J
Res Vet Sci. 1981 May;30(3):298-302.
Suspensions of polymorphonuclear leucocytes (PMN) from fresh bovine blood contained about 90 per cent neutrophils and 10 per cent eosinophils. Such suspensions had no bactericidal activity against Escherichia coli or Staphylococcus aureus, in the absence of an opsonising agent, in Hanks' balanced salt solution. Suitable opsonins were present in dilute (0.5 per cent), heat-inactivated serum or in 5 per cent whey. After incubation with PMN for two hours in the presence of the dilute serum the activity of PMN from several cows against the two organisms was highly correlated (r = 0.98) but varied significantly (P less than 0.001) among the animals from less than 2 to 30 per cent survival of the bacteria. This bactericidal activity did not alter if PMN from a single cow were incubated with sera from other animals, indicating that the critical factor was the cells and not the serum. The ranking of the animals by the bactericidal activity of their PMN was unaltered if 5 per cent whey was used as the opsonin and the leucocytes were sonicated after incubation.
从新鲜牛血中获取的多形核白细胞（PMN）悬液中约含90%的中性粒细胞和10%的嗜酸性粒细胞。在汉克斯平衡盐溶液中，若没有调理素存在，此类悬液对大肠杆菌或金黄色葡萄球菌无杀菌活性。合适的调理素存在于稀释（0.5%）、热灭活的血清或5%的乳清中。在稀释血清存在的情况下，与PMN孵育两小时后，几头奶牛的PMN针对这两种微生物的活性高度相关（r = 0.98），但在不同动物之间差异显著（P小于0.001），细菌存活率从不到2%到30%不等。如果将来自单一奶牛的PMN与其他动物的血清一起孵育，这种杀菌活性不会改变，这表明关键因素是细胞而非血清。若使用5%的乳清作为调理素，并且孵育后对白细胞进行超声处理，根据PMN杀菌活性对动物进行的排名不会改变。
