体外培养的小母牛乳腺巨噬细胞和中性粒细胞对γ干扰素刺激的反应。
Response of heifer mammary gland macrophages and neutrophils to interferon-gamma stimulation in vitro.
作者信息
Quiroga G H, Owens W E, Nickerson S C
机构信息
Hill Farm Research Station, Louisiana State University Agricultural Center, Homer 71040.
出版信息
Can J Vet Res. 1993 Jul;57(3):212-4.
The phagocytic and killing abilities of heifer mammary gland macrophages (M phi) and neutrophils were evaluated after exposure to recombinant bovine interferon-gamma (rBoIFN-gamma) stimulation in vitro. Macrophages or neutrophils were cultured for 2 h with 0, 10(2), 10(4) and 10(5) units rBoIFN-gamma/mL. Phagocytosis assays were performed by incubation with Staphylococcus aureus at a leukocyte:bacteria ratio of 1:10. After 45 min, cells were stained with acridine-orange and phagocytic and killing abilities were determined. Although rBoIFN-gamma had no effect on M phi phagocytic activity, neutrophil phagocytic activity after incubation in 10(4) units rBoIFN-gamma (41.62%) was significantly higher than 0 (25.24%) or 10(2) units rBoIFN-gamma (24.73%). Neutrophil and M Phi killing abilities were not affected by any dose of rBoIFN-gamma. Results suggested that rBoIFN-gamma promoted neutrophil phagocytic activity, but did not affect neutrophil killing or overall M phi function in vitro.
在体外暴露于重组牛干扰素-γ（rBoIFN-γ）刺激后，评估了小母牛乳腺巨噬细胞（M phi）和中性粒细胞的吞噬及杀伤能力。巨噬细胞或中性粒细胞分别与0、10²、10⁴和10⁵单位rBoIFN-γ/mL培养2小时。吞噬试验通过以白细胞：细菌比例1:10与金黄色葡萄球菌孵育进行。45分钟后，细胞用吖啶橙染色并测定吞噬及杀伤能力。尽管rBoIFN-γ对M phi吞噬活性无影响，但在10⁴单位rBoIFN-γ中孵育后的中性粒细胞吞噬活性（41.62%）显著高于0（25.24%）或10²单位rBoIFN-γ（24.73%）。中性粒细胞和M Phi的杀伤能力不受任何剂量rBoIFN-γ的影响。结果表明，rBoIFN-γ促进中性粒细胞吞噬活性，但在体外不影响中性粒细胞杀伤或整体M phi功能。
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