Filev F
Vet Med Nauki. 1980;17(9-10):25-8.
Differentiation was carried out of a total of 170 strains of streptococci that caused mastitis, which were isolated from 3200 milk samples taken from a farm. Ouchterlony's agar gel precipitation method was employed for the serologic typing of the strains along with the Jonagar No. 2 Oxoid and group antisera. The biochemical behaviour of the strains was followed up with regard to the hydrolysis of esculin and sodium hippurate, the fermentation of lactose, sucrose, glucose, trehalose, mannite, and salicin, and the breakdown of arginine. It was found that 17.05 per cent of the isolated mastitis streptococci belonged to group B, 17.64 per cent belonged to group C, and 62.94 per cent--to group C. Untypeable were as many as 2.37 per cent. The behaviour of these three groups of mastitis causative agents with regard to esculin and sodium hippurate proved most stable in the biochemical differentiation of the strains. In this respect the selective TKT agar medium could be used in a mass scale diagnosis for the rapid differentiation of the most frequently isolated mastitis streptococci on the base of the characteristic morphologic traits.
对从一个农场采集的3200份牛奶样本中分离出的总共170株引起乳腺炎的链球菌进行了鉴别。采用奥克特洛尼琼脂凝胶沉淀法，同时使用琼纳加2号奥克托克斯培养基和群抗血清对菌株进行血清学分型。对菌株的生化特性进行了跟踪，包括七叶苷和马尿酸钠的水解、乳糖、蔗糖、葡萄糖、海藻糖、甘露醇和水杨苷的发酵以及精氨酸的分解。结果发现，分离出的乳腺炎链球菌中，17.05%属于B群，17.64%属于C群，62.94%属于C群。无法分型的占2.37%。在菌株的生化鉴别中，这三组乳腺炎病原体在七叶苷和马尿酸钠方面的特性最为稳定。在这方面，选择性TKT琼脂培养基可用于大规模诊断，根据特征性形态学特征快速鉴别最常见的乳腺炎链球菌。
