Todorov D, Iotov I, Iurukov M
Vet Med Nauki. 1977;14(7):75-82.
Comparative studies were carried out on some methods and nutrient media used for the isolation and identification of mastitis causative agents. Milk from normal andmastitis-affected udder quarters was aseptically sampled and treated under various conditions. Individual samples were simultaneously seeded in different media. The following conclusions were drawn: 1. Milk samples for the diagnosis of mastitis of a subclinical character can be taken at the beginning and at the end of milking. 2. Direct seedings of milk have equal diagnostic value as those of the sediment. With the former the process of bacteriologic treatment of the samples is shortened in time, and the isolation and differentiation of the mastitis agents is rendered feasible. 3. At the present etiologic structure of the subclinical types of mastitis in this country most suitable has proved the blood agar medium in which almost all mastitis causative agents can be demonstrated. 4. Kartashova's medium and the TKT-medium possess a pronounced selectivity with special reference to streptococci and have higher diagnostic value for farms where streptococcus mastitis is prevailing.
对用于分离和鉴定乳腺炎病原体的一些方法和营养培养基进行了比较研究。从正常和患乳腺炎的乳房象限无菌采集牛奶，并在各种条件下进行处理。将单个样本同时接种于不同培养基中。得出以下结论：1. 对于诊断亚临床型乳腺炎的牛奶样本，可在挤奶开始时和结束时采集。2. 牛奶直接接种与沉淀物接种具有同等诊断价值。采用前者可缩短样本细菌学处理时间，使乳腺炎病原体的分离和鉴别成为可能。3. 在该国目前亚临床型乳腺炎的病因结构中，血琼脂培养基已被证明是最合适的，几乎所有乳腺炎病原体都能在其中得到显示。4. 卡尔塔绍娃培养基和TKT培养基对链球菌具有显著的选择性，对链球菌性乳腺炎流行的养殖场具有更高的诊断价值。
