Stam H, Hülsmann W C
Ann Nutr Metab. 1981;25(1):11-9. doi: 10.1159/000176474.
Hormonal and metabolic regulation of endogenous triglyceride hydrolysis was studied in triglyceride-enriched hearts obtained from rats fed 3 days with a trierucate-rich diet. Endogenous lipolysis was determined by measuring glycerol release during in vitro perfusion of the hearts. It appeared that there was a direct relation between the contractile state of the heart, the rate of glycerol release in the coronary effluent and the Ca2+ concentration in the perfusion medium. During Ca2+-free perfusion, 2,4-dinitrophenol stimulated oleate oxidation and this, as well as the addition of 2 x 10(-7) M glucagon, induced a marked stimulation of lipolysis. Insulin did not affect glucagon- and norepinphrine-stimulated lipolysis during substrate-free perfusion. The presented experiments point out that in lipid-enriched rat hearts the activity of the tissue lipase may be controlled by the rate of beta-oxidation and re-esterification of the liberated fatty acid, as well as by a shift to utilization of carbohydrate instead of fatty acids for energy supply.
在喂食富含三油酸甘油酯饮食3天的大鼠获取的富含甘油三酯的心脏中，研究了内源性甘油三酯水解的激素和代谢调节。通过测量心脏体外灌注期间甘油的释放来测定内源性脂解作用。结果表明，心脏的收缩状态、冠状动脉流出液中甘油的释放速率与灌注介质中的Ca2+浓度之间存在直接关系。在无Ca2+灌注期间，2,4-二硝基苯酚刺激油酸氧化，并且这以及添加2×10(-7) M胰高血糖素均诱导脂解作用的显著刺激。在无底物灌注期间，胰岛素不影响胰高血糖素和去甲肾上腺素刺激的脂解作用。所呈现的实验指出，在富含脂质的大鼠心脏中，组织脂肪酶的活性可能受释放的脂肪酸的β-氧化和再酯化速率控制，以及受转向利用碳水化合物而非脂肪酸作为能量供应的影响。
