可溶性抗原荧光抗体试验在禽流感诊断中的应用。
The application of the soluble antigen fluorescent antibody test for the diagnosis of avian influenza.
作者信息
Al-Attar M, Nielsen K, Mitchell W R
出版信息
Can J Comp Med. 1981 Apr;45(2):140-6.
The application of the soluble antigen fluorescent test as a tool for serological investigation of influenza type A infection in wild birds was studied. The soluble antigen fluorescent antibody test is basically an indirect fluorescent antibody test except that an artificial matrix of cellulose acetate discs is used as a substrate for antigen and the test results are scanned and recorded by a fluorometer. THe influenza type A soluble antigen fluorescent antibody was obtained from concentrated and detergent disrupted virus particles, absorbed onto cellulose acetate discs. Anti-influenza sera were prepared in pheasants and ducks to A/turkey/Ontario/6118/67 and in pigeons to A/turkey/Ontario/6213/68. The antigen-antibody complex was detected by specific staining with monovalent or polyvalent fluorescein isothiocyanate conjugated rabbit anti-avian immunoglobulins. The soluble antigen fluorescent antibody test is a sensitive technique for the detection of specific influenza A antibodies in several avian species, and could be adapted for use in large scale surveys.
研究了将可溶性抗原荧光试验作为野生鸟类甲型流感感染血清学调查工具的应用。可溶性抗原荧光抗体试验基本上是一种间接荧光抗体试验，只是使用醋酸纤维素圆盘的人工基质作为抗原的底物，并且试验结果由荧光计扫描和记录。甲型流感可溶性抗原荧光抗体是从浓缩和用去污剂破坏的病毒颗粒中获得的，吸附在醋酸纤维素圆盘上。用针对A/火鸡/安大略/6118/67的雉鸡和鸭以及针对A/火鸡/安大略/6213/68的鸽子制备抗流感血清。通过用单价或多价异硫氰酸荧光素偶联的兔抗禽免疫球蛋白进行特异性染色来检测抗原-抗体复合物。可溶性抗原荧光抗体试验是检测几种鸟类中特异性甲型流感抗体的灵敏技术，并且可适用于大规模调查。
Zotero 插件