Comparison of Marion's anaerobic culture/set-A and the Baltimore Biological Laboratory Gaspak anaerobic system in the cultivation and recovery of bacteria from human dental plaque.

Marion's anerobic culture/set-A (Bio-Bag A) and the Baltimore Biological Laboratory (BBL) Gaspak anaerobic system were evaluated for their efficiency in the cultivation and recovery of anaerobes and capnophilic organisms from human dental plaque samples. Both culture systems were relatively efficient in supporting the growth of pure culture of various bacterial species recently isolated from the human dental plaques. When the dental plaque samples were cultured the organisms commonly found were cultivatable in both culture systems. However, the counts and proportions of Fusobacterium nucleatum from the samples were significantly higher in Marion's culture system, whereas the proportions of black-pigmented Bacteroides sp. were significantly higher in the BBL anaerobic system.