用于从牙菌斑中分离粘性放线菌和内氏放线菌的新培养基。
New medium for isolation of Actinomyces viscosus and Actinomyces naeslundii from dental plaque.
作者信息
Kornman K S, Loesche W J
出版信息
J Clin Microbiol. 1978 Jun;7(6):514-8. doi: 10.1128/jcm.7.6.514-518.1978.
Abstract
Metronidazole (10 microgram/ml) and cadmium sulfate (20 microgram/ml) were added to a gelatin-based medium to select for microaerophilic Actinomyces species from dental plaque samples. The new medium (GMC), when incubated anaerobically, allowed 98% recovery of seven pure cultures of Actinomyces viscosus and 73% recovery of eight pure cultures of Actinomyces naeslundii, while suppressing 76% of the total count of other organisms in dental plaque samples. In 203 plaque samples, recoveries of A. viscosus and A. naeslundii on GMC and another selective medium for oral Actinomyces (CNAC-20) were compared. Recovery of A. viscosus was comparable on the two media. Recovery of A. naeslundii was significantly higher on GMC than CNAC-20 (P is less than 0.001), and GMC allowed a more characteristic cell morphology of both organisms. GMC medium appears to be useful for the isolation and presumptive identification of A. viscosus and A. naeslundii from dental plaque.
摘要
将甲硝唑（10微克/毫升）和硫酸镉（20微克/毫升）添加到基于明胶的培养基中，以从牙菌斑样本中筛选微需氧放线菌。新培养基（GMC）在厌氧培养时，可使7株粘性放线菌纯培养物的回收率达到98%，8株内氏放线菌纯培养物的回收率达到73%，同时可抑制牙菌斑样本中其他微生物总数的76%。在203份牙菌斑样本中，比较了GMC和另一种用于口腔放线菌的选择性培养基（CNAC - 20）上粘性放线菌和内氏放线菌的回收率。两种培养基上粘性放线菌的回收率相当。GMC上内氏放线菌的回收率显著高于CNAC - 20（P小于0.001），且GMC可使两种菌呈现更具特征性的细胞形态。GMC培养基似乎有助于从牙菌斑中分离和初步鉴定粘性放线菌和内氏放线菌。
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