Schmatz D M, Murray P K
J Parasitol. 1981 Aug;67(4):517-21.
Trypomastigote or trypomastigote-amastigote populations of Trypanosoma cruzi (Y strain, MERC 2C) entirely free of epimastigotes were obtained from infected muscle cell cultures, and separated from host-cell debris by passage through a DEAE-cellulose column. Approximately 75% of the parasites were recovered and mouse infectivity titrations with postcolumn trypomastigotes showed only a slight reduction in infectivity compared to starting material. Light and electron microscopic examination of material showed a high degree of purity had been achieved by the column procedure. No host-cell debris could be identified in the eluate and parasites were morphologically intact. Enumeration of trypomastigotes and amastigotes in mixed populations before and after column purification showed that there was no preferential loss of either stage and both had the same residence time. This procedure may be used to obtain clean, minimally altered, parasite material free of the invertebrate epimastigote stage and host-cell debris for studies which are sensitive to these contaminants.
从感染的肌肉细胞培养物中获得完全不含前鞭毛体的克氏锥虫（Y株，MERC 2C）的锥鞭毛体或锥鞭毛体-无鞭毛体群体，并通过DEAE-纤维素柱过滤与宿主细胞碎片分离。约75%的寄生虫被回收，柱后锥鞭毛体的小鼠感染性滴定显示，与起始材料相比，感染性仅略有降低。对材料进行光镜和电镜检查表明，通过柱法已达到高度纯度。洗脱液中未发现宿主细胞碎片，寄生虫形态完整。对柱纯化前后混合群体中的锥鞭毛体和无鞭毛体进行计数表明，两个阶段均无优先损失，且停留时间相同。该方法可用于获得不含无脊椎动物前鞭毛体阶段和宿主细胞碎片的、干净的、变化最小的寄生虫材料，用于对这些污染物敏感的研究。
