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使用免疫吸附柱从人胎盘中纯化超氧化物歧化酶。

Purification of superoxide dismutases from human placenta using immunoadsorbent columns.

作者信息

Sugiura M, Adachi T, Inoue H, Ito Y, Hirano K

出版信息

J Pharmacobiodyn. 1981 Apr;4(4):245-50. doi: 10.1248/bpb1978.4.245.

DOI:10.1248/bpb1978.4.245
PMID:7021796
Abstract

Copper and zinc containing-superoxide dismutase (Cu,Zn-SOD), and manganese containing-superoxide dismutase (Mn-SOD) were purified form human placenta with immunoadsorbent columns using anti-human SOD-antibody conjugated Sepharose 4B. The final preparation has a high purity with a good recovery. The Cu,Zn-SOD purified by an immunoadsorbent column had less zinc content and was more labile than the native Cu,Zn-SOD, but this difference disappeared completely by dialyzing the enzyme obtained by immunoadsorbent column against the buffer containing 1mM ZnCl2. Other enzymological properties of two SODs were identical with that of native SODs.

摘要

利用抗人超氧化物歧化酶（SOD）抗体偶联的琼脂糖凝胶4B免疫吸附柱，从人胎盘中纯化出含铜锌超氧化物歧化酶（Cu,Zn-SOD）和含锰超氧化物歧化酶（Mn-SOD）。最终制备物具有高纯度和良好的回收率。通过免疫吸附柱纯化的Cu,Zn-SOD锌含量较低，且比天然Cu,Zn-SOD更不稳定，但通过将免疫吸附柱获得的酶在含有1mM ZnCl2的缓冲液中透析，这种差异完全消失。两种SOD的其他酶学性质与天然SOD相同。

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