Walsh K A, Ericsson L H, Parmelee D C, Titani K
Annu Rev Biochem. 1981;50:261-84. doi: 10.1146/annurev.bi.50.070181.001401.
Although this review cannot possibly cover the myriad of new ideas reported in the analyses of 1100 proteins, it does attempt to highlight procedures and strategies of interest, admittedly with a bias toward examples most familiar to us. It is clear that the scope, efficiency, and sensitivity of the analyses have increased and will continue to do so. Literally any protein sequence can now be solved. The diversity of tactics and strategies adds to the thrill of the chase. If the next few years continue to show the growing interplay between sequence chemists, X-ray crystallographers, and nucleic acid sequencing groups, we may truly begin to understand the meaning underlying the 20-letter hieroglyphics of protein chemistry.
尽管本综述不可能涵盖对1100种蛋白质分析中所报道的无数新观点，但它确实试图突出一些有趣的方法和策略，诚然，这些例子大多是我们比较熟悉的。很明显，分析的范围、效率和灵敏度都有所提高，而且还将继续提高。实际上，现在任何蛋白质序列都可以解析出来。策略和方法的多样性增加了探索的乐趣。如果在接下来的几年里，序列化学家、X射线晶体学家和核酸测序团队之间的相互作用继续增加，我们或许真的能够开始理解蛋白质化学中这20个字母所代表的象形文字背后的含义。
