Chernomordik A B
Antibiotiki. 1981 Jul;26(7):504-6.
A modified procedure for the use of antibiotics in nutrient media for isolation of Shigella is described. The procedure is designated as the method of 3 strips. It implies introduction of 3 different antibiotics, i. e. levomycetin, streptomycin and one of tetracyclines to definite areas of the nutrient medium in a petri dish. The antibiotics diffuse into the medium from 3 strips of filter paper. The greatest part of the medium contains minor amounts of the antibiotics and is used for isolation of the antibiotics sensitive Shigella strains. The procedure is simple and economic. It excludes the most frequent mistakes due to incorrect selection of antibiotics. The preliminary trials showed that the procedure may be used in the routine studies.
本文描述了一种用于在营养培养基中使用抗生素分离志贺氏菌的改良方法。该方法被称为三条带法。它意味着将三种不同的抗生素，即氯霉素、链霉素和四环素中的一种，引入到培养皿中营养培养基的特定区域。抗生素从三条滤纸扩散到培养基中。培养基的大部分区域含有少量抗生素，用于分离对抗生素敏感的志贺氏菌菌株。该方法简单且经济。它避免了因抗生素选择不当而导致的最常见错误。初步试验表明，该方法可用于常规研究。
