Priamukhina N S, Chernomordik A B, Ershov A A, Shcherbinina V V, Grinmaer T V
Antibiotiki. 1979 Oct;24(10):750-5.
A new variant of media with antibiotics for isolation of Shigella, i.e. a method of 2 streaks each containing different antibiotics was tested in analysis of excrements from patients with acute dysentery. It was found that the new method is more effective than the well known method of gradient plates (isolation of Shigella in one series of the experiments amounted to 85.2 and 64.7 per cent respectively, and in the other series of the experiments the respective figures were 95.4 and 89.3 per cent). Its efficiency was lower as compared to the procedure of inoculation onto 2 plates, i.e. onto the media with and without an antibiotic (isolation of Shigella was 67.5 and 77.4 per cent respectively). The new method provided a higher frequency of Shigella isolation as compared to inoculation onto the media without an antibiotic, as well as onto any of the media used with one antibiotic. The method of 2 streaks offers wider possibilities for choosing the antibiotics for adding to the nutrient medium, as well as estimation of the antibioticograms and species structures of Shigella distributed in a concrete area.
一种用于分离志贺氏菌的含抗生素的新型培养基变体，即一种每条划线含不同抗生素的双划线方法，在急性痢疾患者粪便分析中进行了测试。结果发现，新方法比众所周知的梯度平板法更有效（在一系列实验中，志贺氏菌的分离率分别为85.2%和64.7%，在另一系列实验中，相应数字分别为95.4%和89.3%）。与接种到两个平板上的方法相比，即接种到含抗生素和不含抗生素的培养基上，其效率较低（志贺氏菌的分离率分别为67.5%和77.4%）。与接种到不含抗生素的培养基上以及接种到任何一种含单一抗生素的培养基上相比，新方法能提供更高的志贺氏菌分离频率。双划线法为选择添加到营养培养基中的抗生素以及评估分布在特定区域的志贺氏菌的抗生素谱和菌种结构提供了更广泛的可能性。
