Komabayashi T, Sakamoto S, Tsuboi M
Nihon Seirigaku Zasshi. 1978;40(6):127-36.
This investigation was undertaken to clarify the lipolytic response caused by K+ and norepinephrine in brown adipose tissues. 1. Lipolytic response was obviously observed at the concentration of 50 mM in the K+-induced stimulation, and at 1.0 microgram/ml in the norepinephrine-induced stimulation, respectively. 2. K+- and norepinephrine-stimulated lipolysis were inhibited in the Ca2+-deficient tissues, and were inhibited by the addition of Mg2+, Mn2+ and La3+. 3. In the K+-stimulated lipolysis Ca2+ could be substituted only by Sr2+, while in the norepinephrine-stimulated lipolysis the substitution was possible with both Sr2+ and Ba2+. 4. K+- and norepinephrine-stimulated lipolysis were inhibited by the addition of propranolol and procaine. Accordingly, these positive results suggest that K+- and norepinephrine-stimulated lipolysis are dependent on the presence of Ca2+, and are related to the movements of Ca2+ and cyclic AMP system in the cells.
本研究旨在阐明钾离子（K+）和去甲肾上腺素在棕色脂肪组织中引起的脂解反应。1. 在K+诱导的刺激中，浓度为50 mM时明显观察到脂解反应，而去甲肾上腺素诱导的刺激中，浓度为1.0微克/毫升时明显观察到脂解反应。2. 在缺钙组织中，K+和去甲肾上腺素刺激的脂解受到抑制，并且添加镁离子（Mg2+）、锰离子（Mn2+）和镧离子（La3+）也会抑制脂解。3. 在K+刺激的脂解中，钙离子（Ca2+）只能被锶离子（Sr2+）替代，而去甲肾上腺素刺激的脂解中，Sr2+和钡离子（Ba2+）都可以替代Ca2+。4. 添加普萘洛尔和普鲁卡因可抑制K+和去甲肾上腺素刺激的脂解。因此，这些阳性结果表明，K+和去甲肾上腺素刺激的脂解依赖于Ca2+的存在，并且与细胞中Ca2+的移动和环磷酸腺苷（cAMP）系统有关。
