McGuire K L, Vitetta E S
J Immunol. 1981 Oct;127(4):1670-73.
We have used highly specific affinity-purified antibodies against mouse kappa- and lambda chains in conjunction with both immunofluorescence analysis on the FACS and enzymatic radioiodination of murine splenocytes and bone marrow cells. Our results indicate that the kappa/lambda ratio of cell surface immunoglobulin is constant during the ontogenetic development of B cells, i.e., a shift in the kappa/lambda ratio does not occur during B cell maturation. Our results suggest that the events controlling kappa and lambda expression on murine B cells are decided very early in ontogeny, before significant antigenic selection.
我们使用了针对小鼠κ链和λ链的高度特异性亲和纯化抗体，结合在荧光激活细胞分选仪上的免疫荧光分析以及小鼠脾细胞和骨髓细胞的酶促放射性碘化。我们的结果表明，在B细胞的个体发育过程中，细胞表面免疫球蛋白的κ/λ比值是恒定的，即κ/λ比值在B细胞成熟过程中不会发生变化。我们的结果表明，控制小鼠B细胞上κ链和λ链表达的事件在个体发育的早期就已确定，在显著的抗原选择之前。
