Gordon L K
J Immunol Methods. 1981;44(2):241-5. doi: 10.1016/0022-1759(81)90352-5.
A method is presented for repeatedly bleeding rabbits from the central ear artery. The use of hypodermic needle with the hub removed provides a smooth flow of blood and avoids the problem of clot formation in the hub. Blood can be obtained aseptically and free of hemolysis with little risk to the animal. Volumes of 35 ml can be obtained from an initial bleed, increasing to 100 ml from subsequent bleeds. Advantages of the method include reliability, minimal trauma to the animal, and little contamination of the sample.
本文介绍了一种从兔中耳中央动脉反复采血的方法。使用去除针座的皮下注射针可使血液顺畅流动，并避免针座处形成血凝块的问题。可无菌采集血液且无溶血现象，对动物的风险极小。首次采血可获得35毫升血液，后续采血可增至100毫升。该方法的优点包括可靠性高、对动物创伤小以及样本污染少。
