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1
Progress in enzyme immunoassays: production of reagents, experimental design, and interpretation.酶免疫测定的进展：试剂生产、实验设计及结果解读
Bull World Health Organ. 1985;63(4):793-811.
2
Avidin-biotin system in enzyme immunoassays.酶免疫测定中的抗生物素蛋白-生物素系统。
Methods Enzymol. 1990;184:469-81.
3
Improved enzyme immunoassays using biotin-avidin-enzyme complex.使用生物素-抗生物素蛋白-酶复合物的改进型酶免疫测定法。
Arch Pathol Lab Med. 1983 Oct;107(10):514-7.
4
A labeling, detection, and purification system based on 4-hydroxyazobenzene-2-carboxylic acid: an extension of the avidin-biotin system.基于4-羟基偶氮苯-2-羧酸的标记、检测及纯化系统：抗生物素蛋白-生物素系统的扩展
Anal Biochem. 2000 Sep 10;284(2):354-66. doi: 10.1006/abio.2000.4617.
5
Immunohistochemical demonstration of the asialoglycoprotein receptor in rat liver by a sensitive avidin-biotin technique.用灵敏的抗生物素蛋白-生物素技术对大鼠肝脏中去唾液酸糖蛋白受体进行免疫组织化学显示。
J Pathol. 1989 Jul;158(3):247-52. doi: 10.1002/path.1711580313.
6
Overview of antigen detection through enzymatic activity.通过酶活性进行抗原检测概述。
Methods Mol Biol. 2010;588:231-41. doi: 10.1007/978-1-59745-324-0_24.
7
Measurement of serum chorionic gonadotropin by a biotin-avidin labeled enzyme immunoassay.采用生物素-抗生物素蛋白标记酶免疫分析法测定血清绒毛膜促性腺激素。
Ann Clin Lab Sci. 1985 May-Jun;15(3):241-5.
8
[A novel protein microarray detection technique based on biotin-avidin conjugation probe].一种基于生物素-抗生物素蛋白偶联探针的新型蛋白质微阵列检测技术
Sheng Wu Gong Cheng Xue Bao. 2008 Mar;24(3):515-20.
9
Solid-phase biotinylation of antibodies.抗体的固相生物素化
J Mol Recognit. 2004 May-Jun;17(3):268-76. doi: 10.1002/jmr.669.
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New approach to heterogeneous enzyme immunoassays using tagged enzyme-ligand conjugates.使用标记酶-配体缀合物的异质酶免疫测定新方法。
Biochem Biophys Res Commun. 1981 Mar 31;99(2):496-503. doi: 10.1016/0006-291x(81)91772-1.

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Combined administration of serogroup B meningococcal vaccine and conjugated serogroup C meningococcal vaccine is safe and immunogenic in college students.B群脑膜炎球菌疫苗和C群脑膜炎球菌结合疫苗联合接种对大学生而言是安全且具有免疫原性的。
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Enzyme-linked immunosorbent assay based on soluble promastigote antigen detects immunoglobulin M (IgM) and IgG antibodies in sera from cases of visceral and cutaneous leishmaniasis.基于可溶性前鞭毛体抗原的酶联免疫吸附测定可检测内脏利什曼病和皮肤利什曼病患者血清中的免疫球蛋白M（IgM）和免疫球蛋白G（IgG）抗体。
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The significance of blood levels of IgM, IgA, IgG and IgG subclasses in Sudanese visceral leishmaniasis patients.苏丹内脏利什曼病患者血液中IgM、IgA、IgG及IgG亚类水平的意义
Clin Exp Immunol. 1994 Feb;95(2):294-9. doi: 10.1111/j.1365-2249.1994.tb06526.x.
10
Acute respiratory effects in the potato processing industry due to a bioaerosol exposure.马铃薯加工业中因生物气溶胶暴露导致的急性呼吸道影响。
Occup Environ Med. 1994 Feb;51(2):73-8. doi: 10.1136/oem.51.2.73.

本文引用的文献

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A simple chromatographic method for preparation of gamma globulin.一种制备γ球蛋白的简易色谱法。
Proc Soc Exp Biol Med. 1960 Jan;103:250-2. doi: 10.3181/00379727-103-25476.
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Measurement of activity of single molecules of beta-D-galactosidase.β-D-半乳糖苷酶单分子活性的测量
Proc Natl Acad Sci U S A. 1961 Dec 15;47(12):1981-91. doi: 10.1073/pnas.47.12.1981.
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Specificity of the passive hemagglutination test for antibody to rubella virus and the passive hemagglutination response after vaccination.风疹病毒抗体被动血凝试验的特异性及接种疫苗后的被动血凝反应。
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Intestinal brush border aminooligopeptidases: cytosol precursors of the membrane enzyme.肠刷状缘氨基寡肽酶：膜酶的胞质溶胶前体。
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Immune aggregation and not antigen-induced conformational change accounts for enhanced reactivity between immunoglobulin G and protein A.免疫聚集而非抗原诱导的构象变化导致免疫球蛋白G与蛋白A之间反应性增强。
Biochem Biophys Res Commun. 1981 Jun 16;100(3):1326-31. doi: 10.1016/0006-291x(81)91969-0.
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The communication of ELISA data from laboratory to clinician.酶联免疫吸附测定（ELISA）数据从实验室到临床医生的传达。
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The effect of different antibody affinities on ELISA absorbance and titer.不同抗体亲和力对酶联免疫吸附测定（ELISA）吸光度和效价的影响。
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[Horseradish peroxidase: reagent for stopping the catalytic conversion of the substrates H2O2 and 2,2'-azino-di(3-ethyl-benzathiazoline-sulphonic acid-(6)) (ABTS)].[辣根过氧化物酶：用于终止底物过氧化氢（H₂O₂）和 2,2'-叠氮基-二（3-乙基-苯并噻唑啉磺酸-（6））（ABTS）催化转化的试剂]
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Mixing two monoclonal antibodies yields enhanced affinity for antigen.混合两种单克隆抗体可增强对抗原的亲和力。
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10
Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci.金黄色葡萄球菌的A蛋白以及链球菌和肺炎球菌产生的相关免疫球蛋白受体。
Adv Immunol. 1982;32:157-252.

酶免疫测定的进展：试剂生产、实验设计及结果解读

Progress in enzyme immunoassays: production of reagents, experimental design, and interpretation.

作者信息

Kurstak E

出版信息

Bull World Health Organ. 1985;63(4):793-811.

PMID:3910300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2536367/
Abstract

Enzyme immunoassays represent in many cases the preferred procedure for the detection of antigens or corresponding antibodies. However, many of the current procedures are performed suboptimally. This article reviews the available designs, auxiliary recognition systems, production and purification of antibodies, conjugation procedures, solid-phase materials, recording and interpretation of results, and quality control and standardization of procedures to improve the reproducibility of tests.

摘要

在许多情况下，酶免疫测定法是检测抗原或相应抗体的首选方法。然而，目前许多方法的执行情况并不理想。本文综述了现有的设计、辅助识别系统、抗体的生产和纯化、偶联程序、固相材料、结果记录与解读以及程序的质量控制和标准化，以提高检测的可重复性。