酶免疫测定的进展：试剂生产、实验设计及结果解读
Progress in enzyme immunoassays: production of reagents, experimental design, and interpretation.
作者信息
Kurstak E
出版信息
Bull World Health Organ. 1985;63(4):793-811.
Abstract
Enzyme immunoassays represent in many cases the preferred procedure for the detection of antigens or corresponding antibodies. However, many of the current procedures are performed suboptimally. This article reviews the available designs, auxiliary recognition systems, production and purification of antibodies, conjugation procedures, solid-phase materials, recording and interpretation of results, and quality control and standardization of procedures to improve the reproducibility of tests.
摘要
在许多情况下，酶免疫测定法是检测抗原或相应抗体的首选方法。然而，目前许多方法的执行情况并不理想。本文综述了现有的设计、辅助识别系统、抗体的生产和纯化、偶联程序、固相材料、结果记录与解读以及程序的质量控制和标准化，以提高检测的可重复性。
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本文引用的文献
1
A simple chromatographic method for preparation of gamma globulin.一种制备γ球蛋白的简易色谱法。
Proc Soc Exp Biol Med. 1960 Jan;103:250-2. doi: 10.3181/00379727-103-25476.
2
Measurement of activity of single molecules of beta-D-galactosidase.β-D-半乳糖苷酶单分子活性的测量
Proc Natl Acad Sci U S A. 1961 Dec 15;47(12):1981-91. doi: 10.1073/pnas.47.12.1981.
3
Specificity of the passive hemagglutination test for antibody to rubella virus and the passive hemagglutination response after vaccination.风疹病毒抗体被动血凝试验的特异性及接种疫苗后的被动血凝反应。
J Clin Microbiol. 1981 Jan;13(1):226-7. doi: 10.1128/jcm.13.1.226-227.1981.
4
Intestinal brush border aminooligopeptidases: cytosol precursors of the membrane enzyme.肠刷状缘氨基寡肽酶：膜酶的胞质溶胶前体。
Biochemistry. 1980 May 27;19(11):2351-8. doi: 10.1021/bi00552a011.
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Immune aggregation and not antigen-induced conformational change accounts for enhanced reactivity between immunoglobulin G and protein A.免疫聚集而非抗原诱导的构象变化导致免疫球蛋白G与蛋白A之间反应性增强。
Biochem Biophys Res Commun. 1981 Jun 16;100(3):1326-31. doi: 10.1016/0006-291x(81)91969-0.
6
The communication of ELISA data from laboratory to clinician.酶联免疫吸附测定（ELISA）数据从实验室到临床医生的传达。
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