Salley J D, Tassava R A
J Exp Zool. 1981 Feb;215(2):183-9. doi: 10.1002/jez.1402150208.
It is well known that denervated adults newt limbs do not regenerate. Not understood is why denervated newt limb stumps fail to initiate regeneration upon reinnervation. In an effort to define the regeneration limiting factors, we examined the histology of long-term denervated newt limb stumps and tested the effects of various kinds of reinjury. In 5-week denervated limb stumps there were present dedifferentiated cells, differentiated cartilage, and densely packed layered cells. The epidermis covering the limb tip resembled skin epidermis rather than wound epidermis. The kinds of reinjury that were successful in restoring the regeneration capabilities to 5-week denervated limb stumps included; reamputation (100%), a single razor incision (60%), and removal of the healed tissues from the distal limb tip (70%). The results of the study emphasize the importance of both injury and wound epidermis in initiating regeneration.
众所周知，去神经支配的成年蝾螈肢体不会再生。但目前尚不清楚的是，为什么去神经支配的蝾螈肢体残端在重新获得神经支配后仍无法启动再生。为了确定限制再生的因素，我们检查了长期去神经支配的蝾螈肢体残端的组织学，并测试了各种再次损伤的效果。在去神经支配5周的肢体残端中，存在去分化细胞、分化的软骨和密集排列的分层细胞。覆盖肢体末端的表皮类似于皮肤表皮，而不是伤口表皮。成功恢复去神经支配5周的肢体残端再生能力的再次损伤类型包括：再次截肢（100%）、单次剃须刀片切割（60%）以及从肢体末端去除愈合组织（70%）。该研究结果强调了损伤和伤口表皮在启动再生过程中的重要性。
