辐射诱导的DNA双链断裂通过连接和重组过程重新连接。
Radiation induced DNA double strand breaks are rejoined by ligation and recombination processes.
作者信息
Weibezahn K F, Coquerelle T
出版信息
Nucleic Acids Res. 1981 Jul 10;9(13):3139-50. doi: 10.1093/nar/9.13.3139.
Abstract
Using the method of filter elution of double stranded DNA under neutral conditions we have shown that most of gamma-ray induced double strand breaks (DSB) are rejoined in both mammalian and bacterial cells. Rejoining also occurs in the G1 phase in V79 Chinese hamster cells and under different growth conditions. Within 8 minutes at 37 C, half the breaks are rejoined. The rejoining in E. coli is equally fast and depends on the presence of DNA ligase. Some of the breaks in E. coli rejoin slowly, and these require rec+. The non-rejoined DSB are distributed over the DNA without any preference for the nucleosomal or the linker structure in the chromosome. Two kinds of DSB rejoining are discriminated, a fast process of DNA ligation and a slower process involving rec functions.
摘要
我们采用中性条件下双链DNA滤膜洗脱法，证明了在哺乳动物细胞和细菌细胞中，大多数γ射线诱导的双链断裂（DSB）均可重新连接。在V79中国仓鼠细胞的G1期以及不同生长条件下，重新连接也会发生。在37℃下8分钟内，一半的断裂会重新连接。大肠杆菌中的重新连接同样迅速，且依赖于DNA连接酶的存在。大肠杆菌中的一些断裂重新连接缓慢，这些需要rec +。未重新连接的DSB在DNA上随机分布，对染色体中的核小体或连接子结构没有任何偏好。可区分出两种DSB重新连接方式，一种是快速的DNA连接过程，另一种是涉及rec功能的较慢过程。
相似文献
1
Radiation induced DNA double strand breaks are rejoined by ligation and recombination processes.辐射诱导的DNA双链断裂通过连接和重组过程重新连接。
Nucleic Acids Res. 1981 Jul 10;9(13):3139-50. doi: 10.1093/nar/9.13.3139.
2
3
Evidence to support the existence of efficient DNA double-strand break rejoining in a radiosensitive mutant of V79-4 following irradiation with 250 kVp X-rays or neutrons.支持V79-4的辐射敏感突变体在接受250 kVp X射线或中子照射后存在高效DNA双链断裂重新连接的证据。
Mutat Res. 1990 Mar;235(2):41-7. doi: 10.1016/0921-8777(90)90056-b.
4
DNA double-strand break repair in eukaryotic cell lines having radically different radiosensitivities.具有截然不同放射敏感性的真核细胞系中的DNA双链断裂修复
Radiat Res. 1988 Feb;113(2):268-77.
5
Rejoining kinetics of DNA single- and double-strand breaks in normal and DNA ligase-deficient cells after exposure to ultraviolet C and gamma radiation: an evaluation of ligating activities involved in different DNA repair processes.正常细胞和DNA连接酶缺陷细胞在暴露于紫外线C和γ辐射后DNA单链和双链断裂的重新连接动力学：对不同DNA修复过程中涉及的连接活性的评估
Radiat Res. 1999 Apr;151(4):423-32.
6
2-Chlorodeoxyadenosine inhibits the repair of DNA double-strand breaks and does not inhibit the repair of DNA single-strand breaks in X-irradiated Chinese hamster V79 cells.2-氯脱氧腺苷抑制X射线照射的中国仓鼠V79细胞中DNA双链断裂的修复，但不抑制DNA单链断裂的修复。
Chem Biol Interact. 1991;79(3):349-58. doi: 10.1016/0009-2797(91)90114-m.
7
CHO cell repair of single-strand and double-strand DNA breaks induced by gamma- and alpha-radiations.CHO细胞对γ射线和α射线诱导的单链和双链DNA断裂的修复
Basic Life Sci. 1975;5B:665-76. doi: 10.1007/978-1-4684-2898-8_40.
8
X-ray sensitive mutants of Chinese hamster ovary cells defective in double-strand break rejoining.在双链断裂重新连接方面存在缺陷的中国仓鼠卵巢细胞的X射线敏感突变体。
Mutat Res. 1984 Nov-Dec;132(5-6):189-96. doi: 10.1016/0167-8817(84)90037-3.
9
Rejoining of DNA double-strand breaks induced by accelerated nitrogen ions.重离子诱导的DNA双链断裂的修复
Int J Radiat Biol. 1996 Oct;70(4):413-20. doi: 10.1080/095530096144888.
10
Involvement of RecB-mediated (but not RecF-mediated) repair of DNA double-strand breaks in the gamma-radiation production of long deletions in Escherichia coli.RecB介导（而非RecF介导）的DNA双链断裂修复参与大肠杆菌中γ辐射诱导的长片段缺失的产生。
Mutat Res. 1992 Jan;265(1):83-101. doi: 10.1016/0027-5107(92)90041-y.
引用本文的文献
1
DNA repair kinetics in irradiated undifferentiated and terminally differentiated cells.
Radiat Environ Biophys. 1983;22(1):3-19. doi: 10.1007/BF01323757.
2
Relationship between DNA repair and cell recovery: importance of competing biochemical and metabolic processes.
Radiat Environ Biophys. 1985;24(4):271-80. doi: 10.1007/BF01210934.
3
Relationship between double strand break rejoining and G2 block formation in V79 cells.
Radiat Environ Biophys. 1986;25(1):13-21. doi: 10.1007/BF01209680.
4
Gene recombination in X-ray-sensitive hamster cells.X射线敏感仓鼠细胞中的基因重组
Mol Cell Biol. 1987 Apr;7(4):1409-14. doi: 10.1128/mcb.7.4.1409-1414.1987.
5
Promotion of double-strand break repair by human nuclear extracts preferentially involves recombination with intact homologous DNA.人核提取物对双链断裂修复的促进作用主要涉及与完整同源DNA的重组。
Nucleic Acids Res. 1987 Sep 11;15(17):6813-26. doi: 10.1093/nar/15.17.6813.
6
Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.经EcoRI核酸内切酶体内切割后大肠杆菌染色体的修复
Proc Natl Acad Sci U S A. 1989 Apr;86(7):2281-5. doi: 10.1073/pnas.86.7.2281.
7
Induction, repair and biological relevance of radiation-induced DNA lesions in eukaryotic cells.真核细胞中辐射诱导的DNA损伤的诱导、修复及生物学相关性
Radiat Environ Biophys. 1990;29(4):273-92. doi: 10.1007/BF01210408.
8
Directional recombination is initiated at a double strand break in human nuclear extracts.定向重组在人核提取物中的双链断裂处起始。
Nucleic Acids Res. 1992 Feb 11;20(3):501-6. doi: 10.1093/nar/20.3.501.
本文引用的文献
1
Measurement of the kinetics of DNA double strand break repair in Ehrlich ascites tumour cells using the unwinding method.使用解旋法测量艾氏腹水瘤细胞中DNA双链断裂修复的动力学
Int J Radiat Biol Relat Stud Phys Chem Med. 1980 Sep;38(3):335-47. doi: 10.1080/09553008014551691.
2
Negative pion irradiation of mammalian cells. III. A comparative analysis of DNA strand breakage, repair and cell survival after exposure to pi-mesons and X-rays.
Int J Radiat Biol Relat Stud Phys Chem Med. 1980 Oct;38(4):365-71. doi: 10.1080/09553008014551741.
3
The organization and repair of mammalian DNA.哺乳动物DNA的组织与修复。
FEBS Lett. 1974 Aug 25;44(2):153-6. doi: 10.1016/0014-5793(74)80714-3.
4
CHO cell repair of single-strand and double-strand DNA breaks induced by gamma- and alpha-radiations.CHO细胞对γ射线和α射线诱导的单链和双链DNA断裂的修复
Basic Life Sci. 1975;5B:665-76. doi: 10.1007/978-1-4684-2898-8_40.
5
High-LET radiations induce a large proportion of non-rejoining DNA breaks.高传能线密度辐射会导致很大比例的DNA断裂无法重新连接。
Nature. 1977 Apr 14;266(5603):653-5. doi: 10.1038/266653a0.
6
The production and repair of double strand breaks in cells from normal humans and from patients with ataxia telangiectasia.正常人和共济失调毛细血管扩张症患者细胞中双链断裂的产生与修复
Biochim Biophys Acta. 1977 Jan 3;474(1):49-60. doi: 10.1016/0005-2787(77)90213-1.
7
Similar responses to ionizing radiation of fungal and vertebrate cells and the importance of DNA doublestrand breaks.
J Theor Biol. 1978 Apr 6;71(3):339-46. doi: 10.1016/0022-5193(78)90164-9.
8
Molecular recombination and the repair of DNA double-strand breaks in CHO cells.CHO细胞中的分子重组与DNA双链断裂修复
Nucleic Acids Res. 1979 Jul 11;6(9):3145-60. doi: 10.1093/nar/6.9.3145.
9
Repair of radiation-induced DNA double-strand breaks in isolated nuclei of Physarum polycephalum.
Radiat Res. 1979 Aug;79(2):368-76.
10
Repair of DNA double-strand breaks in Escherichia coli, which requires recA function and the presence of a duplicate genome.
J Mol Biol. 1977 Oct 15;116(1):81-98. doi: 10.1016/0022-2836(77)90120-6.
Zotero 插件