Woods R J, Parbhoo S P
Eur Surg Res. 1981;13(4):278-84. doi: 10.1159/000128194.
Isolated hepatocytes prepared from Wistar rats by mechanical means were infused into the liver of congenitally jaundiced Gunn rats. Red cell survival was determined in the recipients and their total plasma bilirubin was measured just before and 20 days after transplantation. Similar measurements were made in transplanted Gunn rats receiving the immunosuppressant drug cyclophosphamide at 5 mg/kg/day and in a group of splenectomized Gun rats. Red cell survival was significantly prolonged in all transplanted rats and in the splenectomised group. Total plasma bilirubin also fell significantly in all three groups by up to 25%, a change we attribute to reduced red cell turnover, haemoglobin synthesis and hence reduced bilirubin synthesis. Blockade of the splenic reticuloendothelial system by hepatocyte debris is suggested as a possible cause.
通过机械方法从Wistar大鼠制备的分离肝细胞被注入先天性黄疸Gunn大鼠的肝脏。测定受体大鼠的红细胞存活率，并在移植前和移植后20天测量其总血浆胆红素。对接受5mg/kg/天免疫抑制剂环磷酰胺的移植Gunn大鼠和一组脾切除的Gunn大鼠进行类似测量。所有移植大鼠和脾切除组的红细胞存活率均显著延长。三组的总血浆胆红素也均显著下降，降幅高达25%，我们将这种变化归因于红细胞周转率、血红蛋白合成降低，从而胆红素合成减少。肝细胞碎片对脾网状内皮系统的阻断被认为是可能的原因。
