Conley F K, Jenkins K A, Remington J S
Hum Pathol. 1981 Aug;12(8):690-8. doi: 10.1016/s0046-8177(81)80170-0.
The peroxidase-antiperoxidase immunohistochemical technique was employed to stain formalin fixed, paraffin embedded tissue sections from three cases of encephalitis caused by Toxoplasma gondii. We studied two cases of congenital infection and one case of acquired toxoplasmosis occurring in an immunocompromised host. The peroxidase-antiperoxidase method was exquisitely sensitive and highly specific and stained both the encysted and tachyzoite forms of the organism, as well as allowing for easy identification of infected cells. In two cases of necrotizing encephalitis--one congenital, the other acquired--widespread dissemination of the Toxoplasma organism throughout the neural parenchyma was visualized using the peroxidase-antiperoxidase stain. Brain biopsy material that had been obtained eight days prior to death in the case of adult acquired toxoplasmosis did not contain any of the characteristic tissue cysts and was not diagnostic for toxoplasmosis by conventional staining techniques. However, peroxidase-antiperoxidase staining of tissue sections from this biopsy unequivocally demonstrated both free tachyzoites and multiple infected cells. Further application of the peroxidase-antiperoxidase method should increase our understanding of the pathology and pathogenesis of toxoplasmic encephalitis as well as allowing timely diagnosis in cases presenting with neurologic symptomatology.
采用过氧化物酶-抗过氧化物酶免疫组织化学技术，对3例由刚地弓形虫引起的脑炎患者经福尔马林固定、石蜡包埋的组织切片进行染色。我们研究了2例先天性感染病例和1例发生在免疫功能低下宿主的获得性弓形虫病病例。过氧化物酶-抗过氧化物酶方法极其灵敏且高度特异，能对该生物体的包囊型和速殖子型进行染色，还便于识别受感染的细胞。在2例坏死性脑炎病例中（1例先天性，另1例获得性），用过氧化物酶-抗过氧化物酶染色可观察到刚地弓形虫生物体在整个神经实质中广泛播散。在成人获得性弓形虫病病例中，死亡前8天获取的脑活检材料不含任何特征性组织囊肿，用传统染色技术无法诊断为弓形虫病。然而，对该活检组织切片进行过氧化物酶-抗过氧化物酶染色明确显示出游离的速殖子和多个受感染细胞。过氧化物酶-抗过氧化物酶方法的进一步应用应能增进我们对弓形虫性脑炎的病理学和发病机制的理解，并能在出现神经系统症状的病例中实现及时诊断。
