Takatsu K, Tominaga A, Hamaoka T
Immunol Lett. 1981 Aug;3(3):137-43. doi: 10.1016/0165-2478(81)90116-4.
TRF responsivity of B-cells from an F1 hybrid of the low-responder strain DBA/2Ha (female) and the high-responder strain BALB/c (male), or their reciprocal F1 hybrid (BALB/c (female) X DBA/2Ha (male)), was analyzed. In every case the B-cells from all of the male mice of the (DBA/2Ha X BALB/c) (DC)F1 hybrid were incapable of responding to TRF, whereas B-cells from female mice of the same litter responded to TRF. In contrast, B-cells from both male and female (BALB/c X DBA/2Ha) (CD)F1 mice were able tp respond to TRF, strongly suggesting that the responsivity of B-cells to TRF is controlled by the X-chromosome of the high-response animals. To substantiate further the evidence for a X-linked B-cell defect in DBA/2Ha mice, we analyzed TRF responsivity of B-cells from back-cross (DCF1 (female) X DBA/2Ha (male)) progeny and from F2 (DCF1 X DCF1) progeny, respectively. In both crosses we observed segregation of TRF responsivity into high-responder and low-responder groups in a nearly 1:1 ratio. These findings further indicated that a major component involved in the regulation of TRF responsivity is carried on the X-chromosome. This component is defective in DBA/2Ha mice, the defect being inherited in a recessive manner.
分析了低反应性品系DBA/2Ha（雌性）与高反应性品系BALB/c（雄性）的F1杂种，或其反交F1杂种（BALB/c（雌性）×DBA/2Ha（雄性））的B细胞对促甲状腺素释放因子（TRF）的反应性。在每种情况下，（DBA/2Ha×BALB/c）（DC）F1杂种所有雄性小鼠的B细胞都不能对TRF作出反应，而同一窝雌性小鼠的B细胞对TRF有反应。相比之下，（BALB/c×DBA/2Ha）（CD）F1雌雄小鼠的B细胞都能够对TRF作出反应，这强烈表明B细胞对TRF的反应性受高反应性动物X染色体的控制。为了进一步证实DBA/2Ha小鼠存在X连锁B细胞缺陷的证据，我们分别分析了回交（DCF1（雌性）×DBA/2Ha（雄性））后代和F2（DCF1×DCF1）后代B细胞对TRF的反应性。在这两种杂交中，我们都观察到TRF反应性以近1:1的比例分离为高反应性和低反应性组。这些发现进一步表明，参与TRF反应性调节的一个主要成分位于X染色体上。该成分在DBA/2Ha小鼠中存在缺陷，且该缺陷以隐性方式遗传。
