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通过间接免疫荧光法检测血清维生素D结合蛋白的脱辅基形式和全蛋白形式与人类淋巴细胞细胞质及细胞膜的结合情况。

Binding of the apo and holo forms of the serum vitamin D-binding protein to human lymphocyte cytoplasm and membrane by indirect immunofluorescence.

作者信息

Constans J, Oksman F, Viau M

出版信息

Immunol Lett. 1981 Aug;3(3):159-62. doi: 10.1016/0165-2478(81)90120-6.

DOI:10.1016/0165-2478(81)90120-6
PMID:7026425
Abstract

The indirect immunofluorescence method is used to study the binding of the serum vitamin D carrier protein (DBP) to lymphocytes. It is shown that in vitro this serum protein will bind to the lymphocyte cytoplasm and intact membrane. This result is in agreement with recent evidence of an actin-binding protein present in the serum. Besides, a difference in the binding to the lymphocyte membrane is observed between the holo forms of the DBP with the different vitamin D derivatives. These findings could be relevant in illuminating the possible role of the DBP in the cellular metabolism of the active metabolites of vitamin D and in the cellular mobility.

摘要

采用间接免疫荧光法研究血清维生素D载体蛋白（DBP）与淋巴细胞的结合情况。结果表明，在体外，这种血清蛋白可与淋巴细胞的细胞质和完整细胞膜结合。这一结果与近期有关血清中存在肌动蛋白结合蛋白的证据相一致。此外，观察到不同维生素D衍生物的DBP全分子形式与淋巴细胞膜的结合存在差异。这些发现可能有助于阐明DBP在维生素D活性代谢产物的细胞代谢及细胞移动性方面可能发挥的作用。

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Binding of the apo and holo forms of the serum vitamin D-binding protein to human lymphocyte cytoplasm and membrane by indirect immunofluorescence.通过间接免疫荧光法检测血清维生素D结合蛋白的脱辅基形式和全蛋白形式与人类淋巴细胞细胞质及细胞膜的结合情况。
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引用本文的文献

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2
Binding of GC (VDBP) to membranes of human B lymphocytes following stripping of extant protein.去除现有蛋白质后，糖皮质激素（维生素D结合蛋白）与人B淋巴细胞膜的结合。
J Endocrinol Invest. 1995 Sep;18(8):630-7. doi: 10.1007/BF03349781.
3
Molecular evaluation of an Alu repeat including a polymorphic variable poly(dA) (AluVpA) in the vitamin D binding protein (DBP) gene.
对维生素D结合蛋白（DBP）基因中包含多态性可变聚腺苷酸（AluVpA）的Alu重复序列进行分子评估。
Hum Genet. 1993 Jan;90(5):526-32. doi: 10.1007/BF00217453.
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[Vitamin D-binding protein in human plasma. Analysis of genetic variation].[人血浆中的维生素D结合蛋白。遗传变异分析]
Naturwissenschaften. 1985 Oct;72(10):519-26. doi: 10.1007/BF00367597.
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Ethnic variation in vitamin D-binding protein (GC): a review of isoelectric focusing studies in human populations.维生素D结合蛋白（GC）的种族差异：人类群体等电聚焦研究综述
Hum Genet. 1986 Apr;72(4):281-93. doi: 10.1007/BF00290950.
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Role of group-specific component (vitamin D binding protein) in clearance of actin from the circulation in the rabbit.群体特异性成分（维生素D结合蛋白）在兔血液循环中肌动蛋白清除中的作用。
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