Parham P
Immunogenetics. 1981;13(6):509-27. doi: 10.1007/BF00343719.
Two monoclonal antibodies (MB40.2 and MB40.3) which are highly specific for HLA-B40 and HLA-B7 were made. They appear to be directed against two separate alloantigenic sites of these HLA molecules. Semiquantitative analysis of the kinetics of antibody binding show that MB40.2 recognizes a site which shows a degree of cross-reactivity with B7 and is specific to some B40 molecules. This antibody also distinguishes between different molecules types as B40. In contrast, MB40.3 recognizes an antigenic determinant which is less variable between B7 and B40 and more closely approximates a public antigen or common antigenic site. This study suggests that the introduction of monoclonal antibodies into MHC serology not only permits but demands a quantitative analysis of these complex systems of homologous but highly polymorphic molecules.
制备了两种对HLA - B40和HLA - B7具有高度特异性的单克隆抗体（MB40.2和MB40.3）。它们似乎针对这些HLA分子的两个不同的同种异体抗原位点。抗体结合动力学的半定量分析表明，MB40.2识别一个与B7有一定交叉反应且对某些B40分子特异的位点。该抗体还能区分不同类型的B40分子。相比之下，MB40.3识别一个在B7和B40之间变化较小且更接近公共抗原或共同抗原位点的抗原决定簇。这项研究表明，将单克隆抗体引入MHC血清学不仅允许而且需要对这些同源但高度多态的分子复杂系统进行定量分析。
