一种用于鉴定鸡淋巴细胞上Ia样同种异体抗原的玫瑰花环试验。

A rosette assay for identification of Ia-like alloantigens on chicken lymphoid cells.

作者信息

Nowak J S, Vainio O, Lassila O, Toivanen P, Gilmour D G

出版信息

J Immunol Methods. 1981;42(3):325-35. doi: 10.1016/0022-1759(81)90161-7.

Abstract

An Ia-rosette assay for the detection of Ia-like alloantigens on the chicken lymphoid cell surface is described. The method is based on the ability of cells treated with alloantiserum to Ia and then with rabbit antiserum against the Fc portion of chicken IgG, to form rosettes with sheep erythrocytes (SRBC) coated with chicken anti-SRBC IgG antibody. Interference with Ia-rosette formation by Fc IgG receptors was eliminated by pronase treatment which removes Fc receptor activity without affecting Ia antigens. The Ia-rosette assay is at least as sensitive as the triple-layer immunofluorescence test for identification of Ia antigens. In addition, the assay allows morphological study and separation of the rosetting cells.

摘要

本文描述了一种用于检测鸡淋巴细胞表面Ia样同种异体抗原的Ia玫瑰花结试验。该方法基于用Ia同种抗血清处理细胞，然后用抗鸡IgG Fc部分的兔抗血清处理后，细胞与包被有鸡抗SRBC IgG抗体的绵羊红细胞（SRBC）形成玫瑰花结的能力。通过链霉蛋白酶处理消除了Fc IgG受体对Ia玫瑰花结形成的干扰，链霉蛋白酶处理可去除Fc受体活性而不影响Ia抗原。Ia玫瑰花结试验在鉴定Ia抗原方面至少与三层免疫荧光试验一样灵敏。此外，该试验允许对形成玫瑰花结的细胞进行形态学研究和分离。