真核生物启动子的缺失作图
Deletion mapping a eukaryotic promoter.
作者信息
Struhl K
出版信息
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4461-5. doi: 10.1073/pnas.78.7.4461.
Abstract
The phenotypes of 24 mutants that successively delete DNA sequences adjacent to the 5' end of the Saccharomyces cerevisiae (yeast) his3 structural gene are described. Deletions retaining greater than 155 base pairs before the mRNA coding sequences are phenotypically indistinguishable from the wild-type his3 allele. Deletions having end points between 113 and 65 base pairs before the transcription initiation site express his3 at reduced levels. Mutations retaining less than 45 base pairs are indistinguishable from null alleles of the his3 locus. These results indicate (i) that a sequence(s) located 113--155 base pairs upstream from the transcribed region is necessary for wild-type expression and (ii) that the T-A-T-A box (a sequence in front of most eukaryotic genes) is not sufficient for wild-type promoter function. Thus, the yeast his3 promoter region appears large when compared with prokaryotic promoters, suggesting that it may be more complex than a simple site of interaction between RNA polymerase and DNA.
摘要
本文描述了24个连续缺失酿酒酵母（酵母）his3结构基因5'端相邻DNA序列的突变体的表型。在mRNA编码序列之前保留大于155个碱基对的缺失在表型上与野生型his3等位基因无法区分。在转录起始位点之前113至65个碱基对之间具有端点的缺失以降低的水平表达his3。保留少于45个碱基对的突变与his3基因座的无效等位基因无法区分。这些结果表明：（i）位于转录区域上游113 - 155个碱基对的一个或多个序列对于野生型表达是必需的；（ii）T - A - T - A框（大多数真核基因前面的一个序列）对于野生型启动子功能是不够的。因此，与原核生物启动子相比，酵母his3启动子区域显得很大，这表明它可能比RNA聚合酶与DNA之间简单的相互作用位点更复杂。
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