Benoist C, Chambon P
Nature. 1981 Mar 26;290(5804):304-10. doi: 10.1038/290304a0.
To investigate the sequences necessary for proper initiation of transcription of SV40 early genes, we have constructed several deletion mutants in the promoter region. The TATA box region is apparently involved in fixing initiation precisely within a narrow area, but is dispensable for gene expression, while the sequences located more than 150 base pairs upstream are indispensable.
为了研究SV40早期基因转录正确起始所必需的序列，我们在启动子区域构建了几个缺失突变体。TATA框区域显然参与在一个狭窄区域内精确确定起始位置，但对于基因表达是可有可无的，而位于上游超过150个碱基对的序列是不可或缺的。
