Campillo J E, Luyckx A S, Lefebvre P J
Horm Metab Res. 1981 Oct;13(10):552-6. doi: 10.1055/s-2007-1019333.
The isolated perfused rat pancreas was used to investigate the effect of extracellular bicarbonate concentration (25 and 40 mmol/l) on the arginine-induced insulin and glucagon release with or without 5.5 mmol/l glucose in the perfusate. In the absence of glucose, the insulin response to arginine was increased at 40 mmol/l bicarbonate, while no potentiation by glucose of the arginine-induced insulin release, at this bicarbonate concentration, was observed. At 40 mmol/l bicarbonate, glucose inhibition of basal glucagon release was abolished. In contrast, the glucagon response to arginine was not affected by the bicarbonate concentration in the perfusion medium. These results confirm that the bicarbonate concentration in the medium markedly influences the insulin release from the isolated perfused rat pancreas, while the glucagon response to arginine seems to be less sensitive to changes in extracellular bicarbonate concentration.
采用离体灌注大鼠胰腺，研究灌流液中细胞外碳酸氢盐浓度（25和40 mmol/L）对精氨酸诱导的胰岛素和胰高血糖素释放的影响，灌流液中有无5.5 mmol/L葡萄糖。在无葡萄糖情况下，40 mmol/L碳酸氢盐时精氨酸诱导的胰岛素反应增强，而在此碳酸氢盐浓度下，未观察到葡萄糖对精氨酸诱导的胰岛素释放有增强作用。在40 mmol/L碳酸氢盐时，葡萄糖对基础胰高血糖素释放的抑制作用消失。相反，灌注液中碳酸氢盐浓度对精氨酸诱导的胰高血糖素反应无影响。这些结果证实，培养基中的碳酸氢盐浓度显著影响离体灌注大鼠胰腺的胰岛素释放，而精氨酸诱导的胰高血糖素反应似乎对细胞外碳酸氢盐浓度的变化不太敏感。
