Effect of Anti-Insulin Receptor Antibody on Insulin Dissociation from IM-9 lymphocytes.

Although anti-insulin receptor antibodies mimic insulin in many of its actions, antireceptor antibodies in serum from a diabetic patient did not share the property of insulin to produce negative cooperativity as defined by the Demeyts experiment. In contrast, an inhibition of insulin dissociation from IM-9 lymphoblastoid cells was observed after exposure to the antireceptor antibody. The capacity of this particular antireceptor antibody to attach to cells whose insulin receptors have been saturated suggests that the antibody may trap insulin by molecular hindrance.