Aitken R J, Richardson D W
J Reprod Fertil. 1981 Nov;63(2):295-307. doi: 10.1530/jrf.0.0630295.
Conditions have been defined under which the sperm binding capacity of the mouse zona pellucida can be measured. As a measure of the concentration of anti-zona antibodies the inhibition of sperm binding appears to be both repeatable and sensitive. Using this technique anti-zona antibody titres were monitored in 6 rats actively immunized with extracts of mouse ovarian tissue. These animals exhibited a rapid rise in anti-zona antibody titre following the induction of immunity, and an associated significant (P less than 0.05) decline in their fertility, both in relation to the proportions of animals exhibiting fertile matings and of matings resulting in conception. Three of the animals exhibited permanent sterility despite repeated exposure to fertile males.
已经确定了能够测量小鼠透明带精子结合能力的条件。作为抗透明带抗体浓度的一种测量方法，精子结合抑制作用似乎具有可重复性且灵敏。利用这项技术，对6只主动用小鼠卵巢组织提取物免疫的大鼠的抗透明带抗体滴度进行了监测。这些动物在免疫诱导后抗透明带抗体滴度迅速升高，并且其生育力出现了相关的显著下降（P小于0.05），这在表现出可育交配的动物比例以及导致受孕的交配方面均如此。尽管反复接触可育雄性，其中3只动物仍表现出永久性不育。
