Bronson R A, Cooper G W, Rosenfeld D L
Fertil Steril. 1982 Dec;38(6):724-9. doi: 10.1016/s0015-0282(16)46701-4.
Heterologous antisperm antibodies induced by immunization with Freund's adjuvants are known to inhibit sperm-zona binding. We have tested whether spontaneously occurring sperm-directed isoantibodies and autoantibodies inhibit the binding of human sperm to the human zona pellucida. Zonae pellucidae were obtained from the ovaries of a woman of reproductive age undergoing surgery for uterine malignancy. Couples at risk for immunity to spermatozoa were screened for humoral antisperm antibodies by immunobead binding. Sera containing head-directed antibodies of IgA and/or IgG classes were selected for passive antibody transfer. Populations of spermatozoa exhibiting differing proportions of antibody-bound sperm were obtained by exposure of antibody-negative sperm of a fertile donor to untreated sera or sera depleted of head-directed antisperm antibodies by prior absorption. Each zona pellucida was challenged sequentially with two populations of sperm. an inverse relationship was found between the number of spermatozoa adherent to the zona and the proportion of sperm exhibiting binding of IgA or IgG immunoglobulins over the head, indicating that the presence of these antibodies in the acrosomal region of the sperm head inhibited their ability to attach to the zona pellucida.
已知用弗氏佐剂免疫诱导产生的异种抗精子抗体可抑制精子与透明带的结合。我们检测了自发产生的精子定向同种抗体和自身抗体是否会抑制人类精子与人类透明带的结合。透明带取自一名因子宫恶性肿瘤接受手术的育龄妇女的卵巢。通过免疫珠结合法对有精子免疫风险的夫妇进行体液抗精子抗体筛查。选择含有IgA和/或IgG类头部定向抗体的血清进行被动抗体转移。通过将一名生育能力正常供体的抗体阴性精子暴露于未经处理的血清或事先吸收去除头部定向抗精子抗体的血清中，获得了显示不同比例抗体结合精子的精子群体。每个透明带依次用两群精子进行挑战。发现附着在透明带上的精子数量与头部显示IgA或IgG免疫球蛋白结合的精子比例呈负相关，这表明精子头部顶体区域存在这些抗体抑制了它们附着于透明带的能力。
