Warweg U, Körting H J
Microsc Acta. 1981 Sep;85(1):7-10.
The influence of embedding media on the fading of FITC-labelled coverslip cultures was tested. It was found, that the value of the fading depend on the pH value of the embedding media. In glycerin-PBS pH 8.5 as embedding media and immediate measuring the fluorescence intensity, the decrease of the fluorescence intensity after 300 seconds blue light excitation was 5%.
测试了包埋介质对异硫氰酸荧光素（FITC）标记的盖玻片培养物褪色的影响。结果发现，褪色值取决于包埋介质的pH值。以pH 8.5的甘油 - 磷酸盐缓冲液（PBS）作为包埋介质并立即测量荧光强度，在蓝光激发300秒后荧光强度的降低为5%。
