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[异硫氰酸荧光素标记盖玻片制剂中褪色的抑制作用]

[Inhibition of fading in FITC-labelled coverslip preparations].

作者信息

Senkpiel K, Klagge E, Körting H J

出版信息

Acta Histochem. 1985;77(2):159-64.

PMID:2420137
Abstract

The influence of anti-fading-substances on the kinetic of fading FITC-labelled coverslip cultures was tested and a quantitative valuation theses compounds was performed. The half-life fluorescence intensity was detected with 2 different methods. The effect of 21 chemical substances was investigated whereof 7 compounds was determined as effective fading-inhibitors. The velocity constants of the 1st order (kapp) of photochemical reaction increase with rising hydrogen activity.

摘要

测试了抗褪色物质对异硫氰酸荧光素(FITC)标记的盖玻片培养物褪色动力学的影响,并对这些化合物进行了定量评估。用两种不同方法检测了半衰期荧光强度。研究了21种化学物质的作用,其中7种化合物被确定为有效的褪色抑制剂。光化学反应一级反应的速度常数(kapp)随氢活性的增加而增大。

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