Senkpiel K, Klagge E, Körting H J
Acta Histochem. 1985;77(2):159-64.
The influence of anti-fading-substances on the kinetic of fading FITC-labelled coverslip cultures was tested and a quantitative valuation theses compounds was performed. The half-life fluorescence intensity was detected with 2 different methods. The effect of 21 chemical substances was investigated whereof 7 compounds was determined as effective fading-inhibitors. The velocity constants of the 1st order (kapp) of photochemical reaction increase with rising hydrogen activity.
测试了抗褪色物质对异硫氰酸荧光素(FITC)标记的盖玻片培养物褪色动力学的影响,并对这些化合物进行了定量评估。用两种不同方法检测了半衰期荧光强度。研究了21种化学物质的作用,其中7种化合物被确定为有效的褪色抑制剂。光化学反应一级反应的速度常数(kapp)随氢活性的增加而增大。