Barber E D, Donish W H, Mueller K R
Mutat Res. 1981 Sep;90(1):31-48. doi: 10.1016/0165-1218(81)90048-3.
We have designed a closed, inert incubation system for testing the mutagenicity of volatile compounds. The containment properties of this system have been investigated using carbon-14 labelled 1,2-dibromoethane. The recovery of this solvent was about 95% following a 48-h incubation at 37 degrees. Using the Ames Salmonella/microsome mutagenicity assay we have determined the mutagenic potency of 10 common halogenated alkane solvents. Of these 10 compounds, only 1,2-dibromoethane and 1-bromo-2-chloroethane give positive results in the standard test procedure, whereas 7 of the 10 give positive results in the closed system. The specificity observed for reversion of the tester strains and the lack of any significant effect of added rat-liver "S9" fractions suggest that these haloalkanes are direct-acting "base-pair" type mutagens. The mutagenic potencies of the 7 positive compounds range from 0.001 revertants per nanomole for 1,2-dichloroethane to 0.172 revertants per nanomole for 1,2-dibromoethane. A minimum or threshold response level for each material has been calculated.
我们设计了一种封闭的惰性培养系统，用于测试挥发性化合物的致突变性。已使用碳-14标记的1,2-二溴乙烷研究了该系统的封闭性能。在37摄氏度下孵育48小时后，这种溶剂的回收率约为95%。我们使用埃姆斯沙门氏菌/微粒体致突变性试验确定了10种常见卤代烷烃溶剂的致突变能力。在这10种化合物中，只有1,2-二溴乙烷和1-溴-2-氯乙烷在标准测试程序中给出阳性结果，而10种中有7种在封闭系统中给出阳性结果。观察到的测试菌株回复突变的特异性以及添加的大鼠肝脏“S9”组分没有任何显著影响，表明这些卤代烷是直接作用的“碱基对”型诱变剂。7种阳性化合物的致突变能力范围从1,2-二氯乙烷的每纳摩尔0.001个回复突变体到1,2-二溴乙烷的每纳摩尔0.172个回复突变体。已计算出每种物质的最低或阈值响应水平。
