Reboul-Salze S, Enjolras F, Gentou C
Pathol Biol (Paris). 1981 Oct;29(8):513-6.
The extraction ot total urinary 17-keto and 17-hydroxy steroids has been effected by a rapid procedure involving pre-packed kieselguhr column chromatography. This work successively studies the correlation of this technique with the classical extraction by mechanical agitation (42 and 61 subjects), its reproducibility, repeatability and recovery of added internal standards. The proposed method seems convenient for routine clinical analysis.
通过一种涉及预装硅藻土柱色谱法的快速程序实现了尿中总 17 - 酮类固醇和 17 - 羟类固醇的提取。这项工作相继研究了该技术与经典机械搅拌提取法（42 名和 61 名受试者）之间的相关性、其重现性、重复性以及添加内标的回收率。所提出的方法似乎便于常规临床分析。
