Chaiken I M
CRC Crit Rev Biochem. 1981;11(3):255-301. doi: 10.3109/10409238109108703.
Semisynthesis provides a flexible approach for using chemical synthesis to produce large, biologically active polypeptides and analogues. Currently developing improvements in the basic methods used, including polypeptide fragmentation, peptide synthesis, and reconstitution of synthetic and native components, make this overall approach applicable to a variety of species. Sequence modification through semisynthesis thus provides a flexible route to explore the code of rules whereby primary structure directs higher order properties of folded conformation and biological function of large peptides and proteins. The fruits of this endeavor, an understanding of how these macromolecules work, and therein, a basis for design of new structures that ultimately may be produced directly or by recombinant DNA methods, have begun to emerge.
半合成提供了一种灵活的方法，利用化学合成来生产大型生物活性多肽及其类似物。目前正在对包括多肽片段化、肽合成以及合成成分与天然成分的重组等基本方法进行改进，这使得这种整体方法适用于多种物种。通过半合成进行序列修饰，为探索规则代码提供了一条灵活的途径，借此一级结构可指导大型肽和蛋白质折叠构象的高阶性质以及生物学功能。这项努力的成果，即对这些大分子如何发挥作用的理解，以及在此基础上设计最终可直接生产或通过重组DNA方法生产的新结构的基础，已开始显现。
