Huberman E
Dev Toxicol Environ Sci. 1980;8:121-32.
A cell-mediated mutagenesis assay was developed to predict the potential carcinogenic hazard of some environmental chemicals. In this assay, cells with appropriate markers for mutagenesis, such as the Chinese hamster V79 cells, are co-cultivated with cells capable of metabolizing chemical carcinogens. Use of this assay made it possible to demonstrate a relationship between the degree of carcinogenicity and mutagenicity of a series of polycyclic hydrocarbons, nitrosamines and aflatoxins, and to establish means to study organ specificity of some chemical carcinogens. However, most shortterm in vitro assays are designed to detect mutagenic activity and therefore do not detect tumor promoting agents which are devoid of this activity. By analyzing various markers of terminal differentiation in cultured human melanoma and myeloid leukemia cells, we have established a relationship between the activity of a series of tumor promoting phorbol diesters in the mouse skin and their ability to induce terminal differentiation. We suggest that measuring alterations in the differentiation. We suggest that measuring alterations in the differentiation characteristics of some cultured cells may represent an approach by which environmental tumor promoting agents can be studied and detected.
开发了一种细胞介导的诱变试验，以预测某些环境化学品的潜在致癌危害。在该试验中，将具有适当诱变标记的细胞，如中国仓鼠V79细胞，与能够代谢化学致癌物的细胞共同培养。使用该试验能够证明一系列多环芳烃、亚硝胺和黄曲霉毒素的致癌性和诱变性之间的关系，并建立研究某些化学致癌物器官特异性的方法。然而，大多数短期体外试验旨在检测诱变活性，因此无法检测到缺乏这种活性的肿瘤促进剂。通过分析培养的人黑色素瘤和髓性白血病细胞中终末分化的各种标记，我们已经建立了一系列肿瘤促进佛波酯在小鼠皮肤中的活性与其诱导终末分化能力之间的关系。我们建议测量分化的变化。我们建议测量某些培养细胞分化特征的变化可能代表一种研究和检测环境肿瘤促进剂的方法。
