Liu D Y, Petschek K D, Remold H G, David J R
J Biol Chem. 1982 Jan 10;257(1):159-62.
We have previously established that preincubation of guinea pig macrophages with crude preparations of macrophage glycolipids enhances their response to a mediator of cellular immunity, migration inhibitory factor. This biologically active moiety found in the extracted material was attributed to a single glycolipid component. This component was purified to homogeneity by phase partition, ion exchange, adsorption, and thin layer chromatography. It migrates in the ganglioside region on thin layer plates, contains sialic acid and sphingosine. There is no apparent identity with any of the common gangliosides possessing the gangliotetraose structure. Homogeneity was demonstrated by rechromatography in four additional solvent systems. These results are discussed in the context of their implications for a deeper understanding of the putative receptor function of this glycolipid.
我们先前已经确定，用巨噬细胞糖脂粗制品对豚鼠巨噬细胞进行预孵育可增强其对细胞免疫介质——迁移抑制因子的反应。在提取物中发现的这种生物活性部分归因于单一糖脂成分。通过相分配、离子交换、吸附和薄层色谱法将该成分纯化至同质。它在薄层板上的神经节苷脂区域迁移，含有唾液酸和鞘氨醇。与任何具有神经节四糖结构的常见神经节苷脂均无明显一致性。通过在另外四种溶剂系统中重新色谱分析证明了其同质性。将根据这些结果对更深入理解这种糖脂的假定受体功能的意义进行讨论。
