Grelck H, Hörchner F, Unterholzner J
Z Parasitenkd. 1981;65(3):277-82. doi: 10.1007/BF00926721.
Groups of eight miniature pigs were infected either with 1,500 embryonated eggs each of Ascaris suum or Toxocara canis or with 1,000 eggs each of both nematodes. Sera were sampled before the infection as well as three and six weeks postinfection and then investigated in the ELISA on microtitre plates against antigens of various developmental stages. When extracts of adult Ascaris and Toxocara worms were used as antigens, distinct reactions were registered both in the infected and in the uninfected control groups. Antigens isolated from either embryonated eggs or larvae of both worms produced distinctly positive reactions with sera of the infected animals, including those pigs with heterologous infections but not with sera from uninfected controls. After saturation with heterologous antigens, the sera showed distinct antibody reactions against homologous antigen extracts and in this way infections with the two nematode species could be serologically differentiated.
将八组小型猪分别感染，每组感染1500枚猪蛔虫或犬弓首蛔虫的感染性虫卵，或者同时感染两种线虫各1000枚虫卵。在感染前以及感染后三周和六周采集血清，然后在酶联免疫吸附测定（ELISA）中用微量滴定板针对不同发育阶段的抗原进行检测。当使用成年猪蛔虫和犬弓首蛔虫的虫体提取物作为抗原时，在感染组和未感染的对照组中均记录到明显反应。从两种线虫的感染性虫卵或幼虫中分离出的抗原与感染动物的血清产生明显的阳性反应，包括那些感染了异种线虫的猪，但未感染动物的血清则无此反应。用异种抗原饱和后，血清对同源抗原提取物呈现明显的抗体反应，从而可以通过血清学方法区分两种线虫的感染。
